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recombinant dickkopf related protein 1  (R&D Systems)


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    Structured Review

    R&D Systems recombinant dickkopf related protein 1
    Recombinant Dickkopf Related Protein 1, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 159 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dickkopf+1+dkk+1/Recombinant+Human+Dkk-1+Protein/pmc12924746-216-17-22
    Average 95 stars, based on 159 article reviews
    recombinant dickkopf related protein 1 - by Bioz Stars, 2026-09
    95/100 stars

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    Recombinant:

    Article Title: Wnt/β-Catenin Signaling Regulates the Proliferation and Differentiation of Mesenchymal Progenitor Cells through the p53 Pathway
    Article Snippet: .. Human Wnt3a recombinant protein (rWnt3a), secreted frizzled-related protein-3 (sFRP-3), and dickkopf-1 (Dkk-1) were purchased from R&D Systems (USA). .. The recombinant rhTGF-β3 protein was purchased from Peprotech (Rocky Hill, NJ, USA).

    Article Title: Wnt/β-catenin signaling regulates the proliferation and differentiation of mesenchymal progenitor cells through the p53 pathway.
    Article Snippet: .. Reagents Human Wnt3a recombinant protein (rWnt3a), secreted frizzledrelated protein-3 (sFRP-3), and dickkopf-1 (Dkk-1) were purchased from R&D Systems (USA). .. The recombinant rhTGF-b3 protein was purchased from Peprotech (Rocky Hill, NJ, USA).

    Article Title: Dysregulation of Frizzled 6 is a critical component of B-cell leukemogenesis in a mouse model of chronic lymphocytic leukemia
    Article Snippet: The data were analyzed using the comparative ΔΔCt method, according to the PCR Array Data Analysis downloaded from the SABiosciences website. .. Splenocytes from an Eμ-TCL1 tumor-bearing mouse (90% CD19 + CD5 + tumor B cells) were cultured in Dulbecco modified Eagle medium plus 10% fetal calf serum (FCS) and 0.5 μM β-mercaptoethanol at 37°C in a humidified 5% CO 2 atmosphere at a concentration of 10 6 cells/mL with or without 75 ng/mL recombinant mouse Wnt3a, 100 ng/mL Wnt5a, 100 ng/mL Dickkopf-1 (DKK-1) (R&D Systems, Minneapolis, MN), or 20 mM LiCl (KCl as the control) for 16, 24, 48, or 72 hours. ..

    Article Title: Wnt3/RhoA/ROCK signaling pathway is involved in adhesion-mediated drug resistance of multiple myeloma in an autocrine mechanism
    Article Snippet: Isotype control mouse IgG1 antibody MOPC21 (Sigma) and rat IgG2a antibody DD13 (Chemicon) was used in the control experiments. .. Recombinant secreted Frizzled-related protein-1 (sFRP-1) and Dickkopf-1 (DKK-1) were obtained from R&D Systems. .. Rho kinase (ROCK) inhibitor Y-27632 and GRGDS (H-Gly-Arg-Gly-Asp-Ser-OH) peptide were purchased from Calbiochem.

    Article Title: Wnt Signaling Promotes Regeneration in the Retina of Adult Mammals
    Article Snippet: .. 5 -Bromo-2 -deoxyuridine (BrdU) (5 g/ml; Sigma) was added to the culture medium for 4 d. Recombinant mouse Wnt3a (10 –100 ng/ml; R & D Systems, Minneapolis, MN), Frizzled-1-cysteine rich domain (Frizzled-1-CRD) (200 ng/ ml; R & D Systems), dickkopf-1 (Dkk-1) (100 ng/ml; R & D Systems), SB216763 [3-(2,4-dichlorophenyl)-4-(1-methyl-1H-indol-3-yl)-1Hpyrrole-2,5-dione] (5 m; Sigma), AR-A014418 [N-(4-methoxybenzyl)N -(5-nitro-1,3-thiazol-2-yl)urea] (5 m; Calbiochem, San Diego, CA), alltrans retinoic acid (RA) (0.3 M; Sigma), and valproic acid (VPA) (1 mM; Sigma) were applied to the culture medium for the indicated time periods. ..

    Article Title: Dysregulation of Frizzled 6 is a critical component of B-cell leukemogenesis in a mouse model of chronic lymphocytic leukemia
    Article Snippet: The data were analyzed using the comparative ΔΔCt method, according to the PCR Array Data Analysis downloaded from the SABiosciences website. .. Proliferation assay Splenocytes from an Eμ-TCL1 tumor-bearing mouse (90% CD19 + CD5 + tumor B cells) were cultured in Dulbecco modified Eagle medium plus 10% fetal calf serum (FCS) and 0.5 μM β-mercaptoethanol at 37°C in a humidified 5% CO 2 atmosphere at a concentration of 10 6 cells/mL with or without 75 ng/mL recombinant mouse Wnt3a, 100 ng/mL Wnt5a, 100 ng/mL Dickkopf-1 (DKK-1) (R&D Systems, Minneapolis, MN), or 20 mM LiCl (KCl as the control) for 16, 24, 48, or 72 hours. ..

    Cell Culture:

    Article Title: Dysregulation of Frizzled 6 is a critical component of B-cell leukemogenesis in a mouse model of chronic lymphocytic leukemia
    Article Snippet: The data were analyzed using the comparative ΔΔCt method, according to the PCR Array Data Analysis downloaded from the SABiosciences website. .. Splenocytes from an Eμ-TCL1 tumor-bearing mouse (90% CD19 + CD5 + tumor B cells) were cultured in Dulbecco modified Eagle medium plus 10% fetal calf serum (FCS) and 0.5 μM β-mercaptoethanol at 37°C in a humidified 5% CO 2 atmosphere at a concentration of 10 6 cells/mL with or without 75 ng/mL recombinant mouse Wnt3a, 100 ng/mL Wnt5a, 100 ng/mL Dickkopf-1 (DKK-1) (R&D Systems, Minneapolis, MN), or 20 mM LiCl (KCl as the control) for 16, 24, 48, or 72 hours. ..

    Article Title: Dysregulation of Frizzled 6 is a critical component of B-cell leukemogenesis in a mouse model of chronic lymphocytic leukemia
    Article Snippet: The data were analyzed using the comparative ΔΔCt method, according to the PCR Array Data Analysis downloaded from the SABiosciences website. .. Proliferation assay Splenocytes from an Eμ-TCL1 tumor-bearing mouse (90% CD19 + CD5 + tumor B cells) were cultured in Dulbecco modified Eagle medium plus 10% fetal calf serum (FCS) and 0.5 μM β-mercaptoethanol at 37°C in a humidified 5% CO 2 atmosphere at a concentration of 10 6 cells/mL with or without 75 ng/mL recombinant mouse Wnt3a, 100 ng/mL Wnt5a, 100 ng/mL Dickkopf-1 (DKK-1) (R&D Systems, Minneapolis, MN), or 20 mM LiCl (KCl as the control) for 16, 24, 48, or 72 hours. ..

    Modification:

    Article Title: Dysregulation of Frizzled 6 is a critical component of B-cell leukemogenesis in a mouse model of chronic lymphocytic leukemia
    Article Snippet: The data were analyzed using the comparative ΔΔCt method, according to the PCR Array Data Analysis downloaded from the SABiosciences website. .. Splenocytes from an Eμ-TCL1 tumor-bearing mouse (90% CD19 + CD5 + tumor B cells) were cultured in Dulbecco modified Eagle medium plus 10% fetal calf serum (FCS) and 0.5 μM β-mercaptoethanol at 37°C in a humidified 5% CO 2 atmosphere at a concentration of 10 6 cells/mL with or without 75 ng/mL recombinant mouse Wnt3a, 100 ng/mL Wnt5a, 100 ng/mL Dickkopf-1 (DKK-1) (R&D Systems, Minneapolis, MN), or 20 mM LiCl (KCl as the control) for 16, 24, 48, or 72 hours. ..

    Article Title: Dysregulation of Frizzled 6 is a critical component of B-cell leukemogenesis in a mouse model of chronic lymphocytic leukemia
    Article Snippet: The data were analyzed using the comparative ΔΔCt method, according to the PCR Array Data Analysis downloaded from the SABiosciences website. .. Proliferation assay Splenocytes from an Eμ-TCL1 tumor-bearing mouse (90% CD19 + CD5 + tumor B cells) were cultured in Dulbecco modified Eagle medium plus 10% fetal calf serum (FCS) and 0.5 μM β-mercaptoethanol at 37°C in a humidified 5% CO 2 atmosphere at a concentration of 10 6 cells/mL with or without 75 ng/mL recombinant mouse Wnt3a, 100 ng/mL Wnt5a, 100 ng/mL Dickkopf-1 (DKK-1) (R&D Systems, Minneapolis, MN), or 20 mM LiCl (KCl as the control) for 16, 24, 48, or 72 hours. ..

    Concentration Assay:

    Article Title: Dysregulation of Frizzled 6 is a critical component of B-cell leukemogenesis in a mouse model of chronic lymphocytic leukemia
    Article Snippet: The data were analyzed using the comparative ΔΔCt method, according to the PCR Array Data Analysis downloaded from the SABiosciences website. .. Splenocytes from an Eμ-TCL1 tumor-bearing mouse (90% CD19 + CD5 + tumor B cells) were cultured in Dulbecco modified Eagle medium plus 10% fetal calf serum (FCS) and 0.5 μM β-mercaptoethanol at 37°C in a humidified 5% CO 2 atmosphere at a concentration of 10 6 cells/mL with or without 75 ng/mL recombinant mouse Wnt3a, 100 ng/mL Wnt5a, 100 ng/mL Dickkopf-1 (DKK-1) (R&D Systems, Minneapolis, MN), or 20 mM LiCl (KCl as the control) for 16, 24, 48, or 72 hours. ..

    Article Title: Dysregulation of Frizzled 6 is a critical component of B-cell leukemogenesis in a mouse model of chronic lymphocytic leukemia
    Article Snippet: The data were analyzed using the comparative ΔΔCt method, according to the PCR Array Data Analysis downloaded from the SABiosciences website. .. Proliferation assay Splenocytes from an Eμ-TCL1 tumor-bearing mouse (90% CD19 + CD5 + tumor B cells) were cultured in Dulbecco modified Eagle medium plus 10% fetal calf serum (FCS) and 0.5 μM β-mercaptoethanol at 37°C in a humidified 5% CO 2 atmosphere at a concentration of 10 6 cells/mL with or without 75 ng/mL recombinant mouse Wnt3a, 100 ng/mL Wnt5a, 100 ng/mL Dickkopf-1 (DKK-1) (R&D Systems, Minneapolis, MN), or 20 mM LiCl (KCl as the control) for 16, 24, 48, or 72 hours. ..

    Control:

    Article Title: Dysregulation of Frizzled 6 is a critical component of B-cell leukemogenesis in a mouse model of chronic lymphocytic leukemia
    Article Snippet: The data were analyzed using the comparative ΔΔCt method, according to the PCR Array Data Analysis downloaded from the SABiosciences website. .. Splenocytes from an Eμ-TCL1 tumor-bearing mouse (90% CD19 + CD5 + tumor B cells) were cultured in Dulbecco modified Eagle medium plus 10% fetal calf serum (FCS) and 0.5 μM β-mercaptoethanol at 37°C in a humidified 5% CO 2 atmosphere at a concentration of 10 6 cells/mL with or without 75 ng/mL recombinant mouse Wnt3a, 100 ng/mL Wnt5a, 100 ng/mL Dickkopf-1 (DKK-1) (R&D Systems, Minneapolis, MN), or 20 mM LiCl (KCl as the control) for 16, 24, 48, or 72 hours. ..

    Article Title: Dysregulation of Frizzled 6 is a critical component of B-cell leukemogenesis in a mouse model of chronic lymphocytic leukemia
    Article Snippet: The data were analyzed using the comparative ΔΔCt method, according to the PCR Array Data Analysis downloaded from the SABiosciences website. .. Proliferation assay Splenocytes from an Eμ-TCL1 tumor-bearing mouse (90% CD19 + CD5 + tumor B cells) were cultured in Dulbecco modified Eagle medium plus 10% fetal calf serum (FCS) and 0.5 μM β-mercaptoethanol at 37°C in a humidified 5% CO 2 atmosphere at a concentration of 10 6 cells/mL with or without 75 ng/mL recombinant mouse Wnt3a, 100 ng/mL Wnt5a, 100 ng/mL Dickkopf-1 (DKK-1) (R&D Systems, Minneapolis, MN), or 20 mM LiCl (KCl as the control) for 16, 24, 48, or 72 hours. ..

    Enzyme-linked Immunosorbent Assay:

    Article Title: Comparison of Efficacy of Romosozumab With Denosumab and Risedronate in Patients Newly Initiating Glucocorticoid Therapy.
    Article Snippet: .. As other biomarkers, serum levels of sclerostin (R and D Systems Cat# DSST00, RRID:AB_2801530), Dickkopf-1 (Dkk-1) (R and D Systems Cat# DY1906, RRID: AB_3661792), receptor activator for nuclear factor κ-B ligand (RANKL) (R and D Systems Cat# DY626, RRID: AB_3661793), osteoprotegerin (OPG) (R and D Systems Cat# DY805, RRID:AB_3083722), and Wnt3a (ABclonal Cat# RK04267, RRID:AB_3661791) were measured using ELISA kits according to the manufacturers’ instructions. ..

    Proliferation Assay:

    Article Title: Dysregulation of Frizzled 6 is a critical component of B-cell leukemogenesis in a mouse model of chronic lymphocytic leukemia
    Article Snippet: The data were analyzed using the comparative ΔΔCt method, according to the PCR Array Data Analysis downloaded from the SABiosciences website. .. Proliferation assay Splenocytes from an Eμ-TCL1 tumor-bearing mouse (90% CD19 + CD5 + tumor B cells) were cultured in Dulbecco modified Eagle medium plus 10% fetal calf serum (FCS) and 0.5 μM β-mercaptoethanol at 37°C in a humidified 5% CO 2 atmosphere at a concentration of 10 6 cells/mL with or without 75 ng/mL recombinant mouse Wnt3a, 100 ng/mL Wnt5a, 100 ng/mL Dickkopf-1 (DKK-1) (R&D Systems, Minneapolis, MN), or 20 mM LiCl (KCl as the control) for 16, 24, 48, or 72 hours. ..



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